Accuracy of the VITEK 2 system to detect glycopeptide resistance in enterococci.
نویسندگان
چکیده
We evaluated the accuracy of the VITEK 2 fully automated system to detect and identify glycopeptide-resistant enterococci (GRE) compared to a reference agar dilution method. The sensitivity of vancomycin susceptibility testing with VITEK 2 for the detection of vanA, vanB, and vanC1 strains was 100%. The sensitivity of vancomycin susceptibility testing of vanC2 strains was 77%. The sensitivity of teicoplanin susceptibility testing of vanA strains was 90%. Of 80 vanC enterococci, 78 (98%) were correctly identified by VITEK 2 as Enterococcus gallinarum/Enterococcus casseliflavus. Since the identification and susceptibility data are produced within 3 and 8 h, respectively, VITEK 2 appears a fast and reliable method for detection of GRE in microbiology laboratories.
منابع مشابه
Validation of VITEK 2 version 4.01 software for detection, identification, and classification of glycopeptide-resistant enterococci.
We evaluated the ability of the new VITEK 2 version 4.01 software to identify and detect glycopeptide-resistant enterococci compared to that of the reference broth microdilution method and to classify them into the vanA, vanB, vanC1, and vanC2 genotypes. Moreover, the accuracy of antimicrobial susceptibility testing with agents with improved potencies against glycopeptide-resistant enterococci ...
متن کاملThe Biomerieux Solution Did You Know? Practical Advice
We evaluated the accuracy of the VITEK 2 fully automated system to detect andidentify glycopeptide-resistant enterococci (GRE) compared to a reference agardilution method. The sensitivity of vancomycin susceptibility testing with VITEK 2for the detection of vanA, vanB, and vanC1 strains was 100%. The sensitivity ofvancomycin susceptibility testing of vanC2 strains was 77%. T...
متن کاملIn vitro susceptibility studies of vancomycin-resistant Enterococcus faecalis.
Vancomycin resistance exhibited by Enterococcus faecalis isolates V583, V586, and V587 is described. The vancomycin MICs ranged from 32 to 64 micrograms/ml. Although resistant to vancomycin, the isolates were susceptible to teicoplanin (MIC, less than or equal to 0.5 micrograms/ml). Such a glycopeptide susceptibility profile has not been previously described for E. faecalis. Time kill studies s...
متن کاملComparison of eight methods to detect vancomycin resistance in enterococci.
A collection of genetically unrelated vancomycin-resistant enterococci (VRE) including 50 vanA, 15 vanB, 50 vanC1, and 30 vanC2 VRE were used to evaluate the accuracy of eight currently available susceptibility test methods (agar dilution, disk diffusion, E-test, agar screen plate, Vitek GPS-TA and GPS-101, and MicroScan overnight and rapid panels). vanA VRE were detected by all methods. vanB V...
متن کاملComparative evaluation of the VITEK 2 Advanced Expert System (AES) in five UK hospitals.
OBJECTIVES We carried out an evaluation of VITEK 2 in five UK laboratories, comparing results with 'gold standard' agar-dilution MIC data, assessing its ability to recognize resistant phenotypes and comparing results with those generated by routine antimicrobial susceptibility testing methods. METHODS Laboratories tested a collection of 82 strains selected on the basis of their challenging an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical microbiology
دوره 39 1 شماره
صفحات -
تاریخ انتشار 2001